1. A rapid sonication based method for preparation of stromal vascular fraction and mesenchymal stem cells from fat tissue

Mohammad Amir Amirkhani, Rashin Mohseni, Masoud Soleimani, Alireza Shoae-Hassani, Mohammad Ali Nilforoushzadeh*

Bioimpacts. 2016;6(2): 99-104. doi: 10.15171/bi.2016.14

PMCID: PMC4981255 PMID: 27525227 Scopus ID: 84983507799

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Original Research

2. A short-term plastic adherence incubation of the stromal vascular fraction leads to a predictable GMP-compliant cell-product

Stephan Born*, Max Johannes Dörfel, Philip Hartjen, Seyed Ali Haschemi Yekani, Julia Luecke, Juliane Katharina Meutsch, Julie Katharina Westphal, Moritz Birkelbach, Robert Köhnke, Ralf Smeets, Michael Krueger

Bioimpacts. 2019;9(3): 161-172. doi: 10.15171/bi.2019.20

PMCID: PMC6726751 PMID: 31508331 Scopus ID: 85071165666

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The purification process for stromal vascular fraction (SVF) can be performed using additional short plastic adherence incubation step (16-20h). This process leads in 24h to a predictable GMP-compliant cell-product. This product is more homogenous than the SVF.

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